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Annex II Entry 1332 07/05/2013

2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products

2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products is listed in Annex II as a prohibited substance for cosmetic products placed on the EU market.

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Prohibited substance— Annex II, EU Regulation 1223/2009
Entry: 1332

Chemical identity and properties

PubChem 2D chemical structure for CAS 2321-07-5
CAS 2321-07-5PubChem CID 16850

Fluorescein

Fluorescein (lactone form) is a xanthene dye that is highly fluorescent, detectable even when present in minute quantities. Used forensically to detect traces of blood, in analytical chemistry as an indicator in silver nitrate titrations and in microscopy. It has a role as a radioopaque medium and a fluorescent dye. It is a polyphenol, a gamma-lactone, a xanthene dye, an oxaspiro compound, an organic heteropentacyclic compound and a member of 2-benzofurans. It is functionally related to a fluorescein (acid form). — ChEBI

IUPAC name
3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
Molecular formula
C20H12O5
Molecular weight
332.3 g/mol
Exact mass
332.06847348 Da
XLogP3
3.4
Topological polar surface area
76.0 Ų
Hydrogen-bond donors
2
Hydrogen-bond acceptors
5
Covalent units
1
Defined stereocentres
0

Also known as

Solvent Yellow 94ResorcinolphthaleinYellow fluoresceinFluoresceineFluorescein acid3,6-FluorandiolD&C Yellow No. 73',6'-Dihydroxyfluoran
16 more reported names
Japan Yellow 201D and C Yellow No. 7C.I. Solvent Yellow 94Soap Yellow FHidacid fluoresceinYellow 7FluoreszeinFluorescein RedD+C Yellow No. 711712 YellowCHEBI:31624Japan Yellow No. 201Zlut kysela 733',6'-FluorandiolKi201NSC-667256
Open full PubChem recordPubChem annotations3D structure image
External database identifiers

Retrieved from NIH PubChem on 2026-09-02 by checksum-valid CAS matching. Chemical properties do not replace the regulatory decision on this page.

PubChem 2D chemical structure for CAS 518-47-8
CAS 518-47-8PubChem CID 10608

Sodium Fluorescein

Fluorescein, disodium salt is an orange-red to dark red powder. Water-soluble form of fluorescein. Odorless and almost tasteless. pH (5% solution) 7.8. May be sensitive to prolonged exposure to light and to heat. — CAMEO Chemicals

IUPAC name
disodium;2-(3-oxido-6-oxoxanthen-9-yl)benzoate
Molecular formula
C20H10Na2O5
Molecular weight
376.3 g/mol
Exact mass
376.03236198 Da
Topological polar surface area
89.5 Ų
Hydrogen-bond donors
0
Hydrogen-bond acceptors
5
Covalent units
3
Defined stereocentres
0

Also known as

Fluorescein disodium saltSoluble fluoresceinUraninUranine WSSResorcinol phthalein sodiumUranine YellowAk-FluorFluorescein, soluble
16 more reported names
Ki202Sodium fluoresceinateFluoresceinum natricum93X55PE38XD&C Yellow No. 8Funduscein-25Ki202(1)Ki202(2)D & C Yellow no. 8disodium 2-(6-oxido-3-oxo-3H-xanthen-9-yl)benzoateCI 45350CI 45350 (Na salt)3',6'-Dihydroxyspiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, disodium saltRetinofluorGlostripsBioglo
Open full PubChem recordPubChem annotations3D structure image
External database identifiers

Retrieved from NIH PubChem on 2026-09-02 by checksum-valid CAS matching. Chemical properties do not replace the regulatory decision on this page.

Record details

Chemical name

2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products

Regulatory information

Regulation

(EC) 2013/344

See every entry this act set

Identified ingredients

Key data

CAS
2321-07-5 -- 518-47-8
EC
219-031-8 -- 208-253-0
Annex
II
Entry number
1332

Inventory links for 2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products

Inventory Restriction reference
Ref 54164

ACID YELLOW 73

CAS: 2321-07-5
EC: 219-031-8
Inventory Restriction reference
Ref 32767

CI 45350

CAS: 518-47-8 / 2321-07-5 / 6417-85-2
EC: 208-253-0 / 219-031-8 / 229-143-9

Direct matches are limited for this record, so nearby entries from Annex II are shown as well.

Annex IV 74
Shared CAS number

CI 45350

CAS: 518-45-6; 518-47-8
EC: -; 208-253-0

What Annex II means for 2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products

2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products is listed as a prohibited substance under Annex II of EU Cosmetics Regulation 1223/2009 and must not be intentionally added. Article 17 separately addresses unintended small quantities that are technically unavoidable under good manufacturing practice and compatible with the Article 3 safety requirement.

Formulators must screen their ingredients and raw materials to ensure none contain this substance. Suppliers should provide documentation confirming absence when requested.

This record is linked to 3 CosIng inventory ingredients for name and identifier cross-reference. The page also surfaces 12 related annex records with overlapping identifiers, annex logic or naming patterns.

Compliance checklist

  • Verify this substance is absent from all raw materials and finished formulations.
  • Request absence confirmation or CoA from raw material suppliers.
  • Document the screening result in the Product Information File (PIF).

Frequently asked questions

Yes. 2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products is listed in Annex II of EU Cosmetics Regulation 1223/2009 and is prohibited as an intentionally added cosmetic ingredient. Article 17 provides a narrow exception for unintended small quantities that are technically unavoidable under good manufacturing practice, provided the product remains safe under Article 3.

CAS 2321-07-5 -- 518-47-8 is the unique numerical identifier assigned to 2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products by the Chemical Abstracts Service. In cosmetic compliance work, the CAS number is used to cross-reference the substance across regulatory databases, raw material specifications and safety assessment reports. Multiple EU annex entries may share the same CAS number if the same substance appears under different conditions or in different annexes.

Use the INCI Checker tool on this site to paste your full ingredient list (INCI) and scan it against all Annex II–VI entries. The tool highlights any ingredients that match restricted, prohibited or allergen-flagged records. This is useful for rapid compliance screening of finished product formulas or raw material declarations.
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Source note for Annex II record 2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products

This page summarizes one Annex II record for 2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products from public European Commission cosmetic materials and adds linked inventory or identifier matches when available.

Use this record page to review Annex II conditions for 2-(6-Hydroxy-3-oxo-(3H)xanthen-9-yl)benzoic acid; Fluorescein and its disodium salt (Acid Yellow 73 sodium salt; CI 45350), when used as a substance in hair dye products, but confirm any final regulatory decision against the current official annex wording and source files.

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