Annex II Entry 132 15/10/2010

[Oxalylbis(iminoethylene)]bis[(o-chlorobenzyl)diethylammonium] salts, e.g. ambenonium chloride (INN)

Q: How do I check if my product formula contains [Oxalylbis(iminoethylene)]bis[(o-chlorobenzyl)diethylammonium] salts, e.g. ambenonium chloride (INN)?

Use the INCI Checker tool on CosIng Checker to paste your full ingredient list and scan it against all Annex II–VI entries. The tool highlights any ingredients that match restricted, prohibited or allergen-flagged records.

[Oxalylbis(iminoethylene)]bis[(o-chlorobenzyl)diethylammonium] salts, e.g. ambenonium chloride (INN) is listed in Annex II as a prohibited substance for cosmetic products placed on the EU market.

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Prohibited substance— Annex II, EU Regulation 1223/2009

Entry: 132

Chemical identity and properties

PubChem 2D chemical structure for CAS 115-79-7

CAS 115-79-7PubChem CID 8288

Ambenonium Chloride

Ambenonium chloride is a symmetrical oxalamide-based bis-quaternary ammonium salt having ethyl and 2-chlorobenzyl groups attached to the nitrogens. It has a role as a neurotransmitter agent and an EC 3.1.1.8 (cholinesterase) inhibitor. It is a quaternary ammonium salt and an organic chloride salt. — ChEBI

IUPAC name: (2-chlorophenyl)methyl-[2-[[2-[2-[(2-chlorophenyl)methyl-diethylazaniumyl]ethylamino]-2-oxoacetyl]amino]ethyl]-diethylazanium dichloride

Molecular formula: C28H42Cl4N4O2
Molecular weight: 608.5 g/mol
Exact mass: 608.203237 Da
Topological polar surface area: 58.2 Å²
Hydrogen-bond donors: 2
Hydrogen-bond acceptors: 4
Covalent units: 3
Defined stereocentres: 0

Also known as

MytelaseAmbenonii chloridumAmbestigmin chlorideOxazylumMisuranOxazylCloruro de ambenonioChlorure d'ambenonium

16 more reported names

Win 807751FOB87G3IN,N'-Bis(2-diethylaminoethyl)oxamide bis(2-chlorobenzyl chloride)(Oxalylbis(iminoethylene))bis((o-chlorobenzyl)diethylammonium) dichlorideCHEBI:2628Benzenemethanaminium, N,N'-((1,2-dioxo-1,2-ethanediyl)bis(imino-2,1-ethanediyl))bis(2-chloro-N,N-diethyl-, dichlorideChloride, Ambenonium[Oxalylbis(iminoethylene)]bis[(O-chlorobenzyl)diethylammonium] dichlorideambAnonium (cation)RefChem:57385Benzenemethanaminium, N,N'-((1,2-dioxo-1,2-ethanediyl)bis(imino-2,1-ethanediyl))bis(2-chloro-N,N-diethyl)-, dichlorideN07AA30(Oxalylbis(iminoethylene))bis((o-chlorobenzyl)diethyl-ammonium), Dichloride2,2'-((1,2-Dioxoethane-1,2-diyl)diimino)bis(N-(2-chlorobenzyl)-N,N-diethylethylaminium) dichloride204-107-5N,N'-((1,2-Dioxo-1,2-ethanediyl)bis(imino-2,1-ethanediyl))bis(2-chloro-N,N-diethyl-benzenemethanaminium), Dichloride

Open full PubChem record PubChem annotations 3D structure image

External database identifiers

ChEBI:CHEBI:2628
ChEMBL:CHEMBL1200541
EPA DTXSID:DTXSID3022582
PubMed:33844597
PubMed:11132996
PubMed:11099780
PubMed:8010419
PubMed:3912090

Evidence from additional scientific databases

NIH PubChem PUG-View

Exact identifier match8288

Attributed physical-property, hazard, environmental and use annotations.

Carcinogen Classification: No indication of carcinogenicity to humans (not listed by IARC). Source: Toxin and Toxin Target Database (T3DB)

Exposure Routes: Oral - poorly absorbed from the gastrointestinal tract. Source: Toxin and Toxin Target Database (T3DB)

Signal: Danger Source: European Chemicals Agency (ECHA)
GHS Hazard Statements: H300 (100%): Fatal if swallowed [Danger Acute toxicity, oral] Source: European Chemicals Agency (ECHA)
Precautionary Statement Codes: P264, P270, P301+P316, P321, P330, P405, and P501 (click each P-code to see the statement) Source: European Chemicals Agency (ECHA)
ECHA C&L Notifications Summary: Aggregated GHS information provided per 38 reports by companies from 1 notifications to the ECHA C&L Inventory.; Information may vary between notifications depending on impurities, additives, and other factors. The percentage value in parenthesis indicates the notified classification ratio from companies that provide hazard codes. Only hazard codes with percentage values above 10% are shown. For more detailed information, please visit ECHA C&L website. Source: European Chemicals Agency (ECHA)

Health Effects: Acute exposure to cholinesterase inhibitors can cause a cholinergic crisis characterized by severe nausea/vomiting, salivation, sweating, bradycardia, hypotension, collapse, and convulsions. Increasing muscle weakness is a possibility and may result in death if respiratory muscles are involved. Accumulation of ACh at motor nerves causes overstimulation of nicotinic expression at the neuromuscular junction. When this occurs symptoms such as muscle weakness, fatigue, muscle cramps, fasciculation, and paralysis can be seen. When there is an accumulation of ACh at autonomic ganglia this causes overstimulation of nicotinic expression in the sympathetic system. Symptoms associated with this are hypertension, and hypoglycemia. Overstimulation of nicotinic acetylcholine receptors in the central nervous system, due to accumulation of ACh, results in anxiety, headache, convulsions, ataxia, depression of respiration and circulation, tremor, general weakness, and potentially coma. When there is expression of muscarinic overstimulation due to excess acetylcholine at muscarinic acetylcholine receptors symptoms of visual disturbances, tightness in chest, wheezing due to bronchoconstriction, incre Source: Toxin and Toxin Target Database (T3DB)

Toxicity Summary: Ambenonium is a cholinesterase or acetylcholinesterase (AChE) inhibitor. A cholinesterase inhibitor (or 'anticholinesterase') suppresses the action of acetylcholinesterase. Because of its essential function, chemicals that interfere with the action of acetylcholinesterase are potent neurotoxins, causing excessive salivation and eye-watering in low doses, followed by muscle spasms and ultimately death. Nerve gases and many substances used in insecticides have been shown to act by binding a serine in the active site of acetylcholine esterase, inhibiting the enzyme completely. Acetylcholine esterase breaks down the neurotransmitter acetylcholine, which is released at nerve and muscle junctions, in order to allow the muscle or organ to relax. The result of acetylcholine esterase inhibition is that acetylcholine builds up and continues to act so that any nerve impulses are continually transmitted and muscle contractions do not stop. Among the most common acetylcholinesterase inhibitors are phosphorus-based compounds, which are designed to bind to the active site of the enzyme. The structural requirements are a phosphorus atom bearing two lipophilic groups, a leaving group (such as a hal Source: Toxin and Toxin Target Database (T3DB)

Uses: Ambenonium is used to treat muscle weakness due to muscle disease (myasthenia gravis). Source: Toxin and Toxin Target Database (T3DB)

Mapped by: Existing checksum-valid CAS to unique PubChem CID match. Retrieved: 2026-08-31. Open source record

Scientific classifications and hazard annotations provide context and do not replace the market-specific regulatory decision shown above.

Additional source coverage and match status

Source	Result	Checked
NIH PubChem PUG-View	Exact match	2026-08-31

A recorded no-match is useful evidence that the source was checked; it is not a claim that the substance does not exist elsewhere.

Retrieved from NIH PubChem on 2026-09-02 by checksum-valid CAS matching. Chemical properties do not replace the regulatory decision on this page.

Record details

Chemical name

[Oxalylbis(iminoethylene)]bis[(o-chlorobenzyl)diethylammonium] salts, e.g. ambenonium chloride (INN)

Regulatory information

Regulation

(EC) 2009/1223

See every entry this act set

Key data

CAS

115-79-7

204-107-5

Annex

Entry number

132

Direct matches are limited for this record, so nearby entries from Annex II are shown as well.

Annex II 131