Why does CAS 59-05-2 appear in more than one EU cosmetic annex?

A substance identified by CAS 59-05-2 may appear in multiple EU cosmetic regulation annexes when it is subject to different conditions in different contexts — for example, restricted under Annex III with specific conditions, while also appearing in Annex IV as an approved colorant under separate entry conditions. Each entry must be reviewed individually.

How do I check if my formula contains a substance with CAS 59-05-2?

Use the INCI Checker on CosIng Checker with INCI names, CAS numbers or EC numbers. Paste CAS 59-05-2 to screen it directly, then review every linked annex record and supplier document.

CAS 1 record

CAS 59-05-2

Q: How do I use CAS 59-05-2 for compliance screening?

Cross-reference CAS 59-05-2 against your raw material specification sheets and CoA documents. When it appears in Annex II, verify absence. When it appears in Annex III, verify that concentration limits and product type conditions are met. All annex entries sharing this CAS number are listed on this page.

CAS 59-05-2 matches 1 EU annex record across Annex II–VI of Regulation 1223/2009.

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Chemical identity and properties

PubChem 2D chemical structure for CAS 59-05-2

CAS 59-05-2PubChem CID 126941

Methotrexate

Methotrexate is a member of pteridines, a monocarboxylic acid amide and a dicarboxylic acid. It has a role as an antimetabolite, an antineoplastic agent, an immunosuppressive agent, an antirheumatic drug, a dermatologic drug, an EC 1.5.1.3 (dihydrofolate reductase) inhibitor, an abortifacient and a DNA synthesis inhibitor. It is functionally related to a L-glutamic acid. It is a conjugate acid of a methotrexate(1-). — ChEBI

IUPAC name: (2S)-2-[[4-[(2,4-diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid

Molecular formula: C20H22N8O5
Molecular weight: 454.4 g/mol
Exact mass: 454.17131583 Da
XLogP3: -1.8
Topological polar surface area: 211.0 Å²
Hydrogen-bond donors: 5
Hydrogen-bond acceptors: 12
Covalent units: 1
Defined stereocentres: 1

Also known as

RheumatrexAmethopterinMetatrexanHdmtxLedertrexateMethylaminopterinumMethotrexatumMetotrexato

16 more reported names

AntifolanRasuvoEmtexateL-AmethopterinAmethopterineNSC-740MaxtrexFarmitrexatMedsatrexateMethoblastinMethotextrateMethotrexatBrimexateEmthexatEmthexateFauldexato

Open full PubChem record PubChem annotations 3D structure image

External database identifiers

ChEBI:CHEBI:44185
ChEMBL:CHEMBL34259
EPA DTXSID:DTXSID4020822
PubMed:32020584
PubMed:30415610
PubMed:29268019
PubMed:28117352
PubMed:27379764
PubMed:27210722
PubMed:24284432
PubMed:23895801
PubMed:22968027
PubMed:22702814
PubMed:22639782
PubMed:22587841

Retrieved from NIH PubChem on 2026-08-31 by checksum-valid CAS matching. Chemical properties do not replace the regulatory decision on this page.

Records for CAS 59-05-2

1 total

Annex

Entry

Methotrexate (INN)

200-413-8

How CAS 59-05-2 is used on this site

CAS 59-05-2 is the Chemical Abstracts Service identifier used to pin this page to a specific substance. In the current dataset, 1 EU annex record shares this identifier, which helps you cross-check whether the same substance appears under one regulatory context or several.

Use this page when a supplier specification, SDS or raw-material dossier gives you CAS 59-05-2 and you need to see every linked Annex II–VI record in one place. The entry cards above are the quickest route to concentration limits, warnings, annex placement and product-type conditions.

Frequently asked questions

A substance identified by CAS 59-05-2 may appear in multiple EU cosmetic regulation annexes when it is subject to different conditions in different contexts. For example, a substance might be restricted under Annex III with specific conditions, and the same CAS might also appear in Annex IV as an approved colorant under separate entry conditions. Each entry must be reviewed individually.

Cross-reference the CAS number against your raw material specification sheets and CoA documents. When a CAS appears in Annex II, verify absence. When it appears in Annex III, verify that concentration limits and product type conditions are met in your formulation. All annex entries sharing this CAS number are listed above.

A CAS number is assigned by the Chemical Abstracts Service (USA) and is globally recognised. An EC number is a European Commission identifier from inventories such as EINECS, ELINCS or the NLP list. Both identifiers may appear in EU cosmetic annex entries. Some substances have both, some have only one, and some older entries may have neither.

The INCI Checker accepts ingredient names, CAS numbers and EC numbers. Check this CAS number directly, then verify the result against supplier specification sheets and every linked annex condition.

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Tools & next steps

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