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CAS 1 record

CAS 477-73-6

CAS 477-73-6 matches 1 EU annex record across Annex II–VI of Regulation 1223/2009.

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Chemical identity and properties

PubChem 2D chemical structure for CAS 477-73-6
CAS 477-73-6PubChem CID 2723800

C.I. Basic Red 2

Safranin O is an organic chloride salt having 3,7-diamino-2,8-dimethyl-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria. It has a role as a fluorochrome and a histological dye. It contains a 3,7-diamino-2,8-dimethyl-5-phenylphenazin-5-ium. — ChEBI

IUPAC name
3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine chloride
Molecular formula
C20H19ClN4
Molecular weight
350.8 g/mol
Exact mass
350.1298243 Da
Topological polar surface area
68.8 Ų
Hydrogen-bond donors
2
Hydrogen-bond acceptors
4
Covalent units
2
Defined stereocentres
0

Also known as

BASIC RED 2SafraninSafranine OSafranine TSafranin TGossypimineSafranine ZhSafranine A
16 more reported names
Safranine THSafranineSafranine BSafranine GSafranine JSafranine YSafranine GFSafranine OKSafranine TNSafranine TSSafranine YNTolusafranineHidaco safranineMitsui safranineBrilliant safranine GCalcozine Red Y
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External database identifiers

Retrieved from NIH PubChem on 2026-08-31 by checksum-valid CAS matching. Chemical properties do not replace the regulatory decision on this page.

Records for CAS 477-73-6

1 total

How CAS 477-73-6 is used on this site

CAS 477-73-6 is the Chemical Abstracts Service identifier used to pin this page to a specific substance. In the current dataset, 1 EU annex record shares this identifier, which helps you cross-check whether the same substance appears under one regulatory context or several.

Use this page when a supplier specification, SDS or raw-material dossier gives you CAS 477-73-6 and you need to see every linked Annex II–VI record in one place. The entry cards above are the quickest route to concentration limits, warnings, annex placement and product-type conditions.

Frequently asked questions

A substance identified by CAS 477-73-6 may appear in multiple EU cosmetic regulation annexes when it is subject to different conditions in different contexts. For example, a substance might be restricted under Annex III with specific conditions, and the same CAS might also appear in Annex IV as an approved colorant under separate entry conditions. Each entry must be reviewed individually.

Cross-reference the CAS number against your raw material specification sheets and CoA documents. When a CAS appears in Annex II, verify absence. When it appears in Annex III, verify that concentration limits and product type conditions are met in your formulation. All annex entries sharing this CAS number are listed above.

A CAS number is assigned by the Chemical Abstracts Service (USA) and is globally recognised. An EC number is a European Commission identifier from inventories such as EINECS, ELINCS or the NLP list. Both identifiers may appear in EU cosmetic annex entries. Some substances have both, some have only one, and some older entries may have neither.

The INCI Checker accepts ingredient names, CAS numbers and EC numbers. Check this CAS number directly, then verify the result against supplier specification sheets and every linked annex condition.
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