
2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-3-(2-(4-nitrophenyl)diazenyl)-6-(2-phenyldiazenyl)-, sodium salt (1:?)
Amido Black 10B is an organic sodium salt resulting from the formal condensation of Amido Black 10B (acid form) with two equivalents of sodium hydroxide. It has a role as a histological dye. It contains a 4-amino-5-hydroxy-3-[(p-nitrophenyl)azo]-6-(phenylazo)-naphthalene-2,7-disulfonate. — ChEBI
- IUPAC name
- disodium;4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonate
- Molecular formula
- C22H14N6Na2O9S2
- Molecular weight
- 616.5 g/mol
- Exact mass
- 616.00590696 Da
- Topological polar surface area
- 273.0 Ų
- Hydrogen-bond donors
- 2
- Hydrogen-bond acceptors
- 14
- Covalent units
- 3
- Defined stereocentres
- 0
Also known as
16 more reported names
External database identifiers
- ChEBI:CHEBI:86230
- EPA DTXSID:DTXSID1020934
- EPA DTXSID:DTXSID1024415
Evidence from additional scientific databases
EMBL-EBI ChEBI
Amido Black 10B
An organic sodium salt resulting from the formal condensation of Amido Black 10B (acid form) with two equivalents of sodium hydroxide.
- Formula
- C22H14N6O9S2.2Na
- Average mass
- 616.501 g/mol
- Monoisotopic mass
- 616.00591 Da
- Formal charge
- 0
- organic sodium salt — is a (CHEBI:38700)
- histological dye — has role (CHEBI:77178)
- 4-amino-5-hydroxy-3-[(p-nitrophenyl)azo]-6-(phenylazo)-naphthalene-2,7-disulfonate — has part (CHEBI:86227)
- histological dye — has role (CHEBI:77178)
- organic sodium salt — is a (CHEBI:38700)
Additional curated names
Cross-references from this source
- CAS: 1064-48-8 — ChemIDplus
- MANUAL_X_REF: Amido_black_10B — Wikipedia
Mapped by: Exact CAS cross-reference in the ChEBI compound record. Source updated: 2015-06-29. Retrieved: 2026-08-31. Open source record
NIH PubChem PUG-View
Attributed physical-property, hazard, environmental and use annotations.
- Physical Description: A group of dark blue or black dyes; [Hawley] Black powder; [MSDSonline] There are water-soluble, ethanol-soluble, and fat-soluble nigrosines; [Ullmann] Source: Haz-Map, Information on Hazardous Chemicals and Occupational Diseases
- Solubility: SOME SOL IN WATER, ALC, OIL /NIGROSINES/ Source: Hazardous Substances Data Bank (HSDB)
- GHS Hazard Statements: Not Classified; Reported as not meeting GHS hazard criteria by 283 of 315 companies Source: European Chemicals Agency (ECHA)
- ECHA C&L Notifications Summary: Aggregated GHS information provided per 315 reports by companies from 4 notifications to the ECHA C&L Inventory.; Reported as not meeting GHS hazard criteria per 283 of 315 reports by companies.; There are 2 notifications provided by 32 of 315 reports by companies with hazard statement code(s).; Information may vary between notifications depending on impurities, additives, and other factors. The percentage value in parenthesis indicates the notified classification ratio from companies that provide hazard codes. Only hazard codes with percentage values above 10% are shown. For more detailed information, please visit ECHA C&L website. Source: European Chemicals Agency (ECHA)
- Note: This chemical does not meet GHS hazard criteria for 100% (3 of 3) of all reports. Source: European Chemicals Agency (ECHA)
- GHS Hazard Statements: Not Classified; Reported as not meeting GHS hazard criteria by 3 of 3 companies. For more detailed information, please visit ECHA C&L website. Source: European Chemicals Agency (ECHA)
- ECHA C&L Notifications Summary: Aggregated GHS information provided per 3 reports by companies from 1 notifications to the ECHA C&L Inventory.; Reported as not meeting GHS hazard criteria per 3 of 3 reports by companies.; There are 0 notifications provided by 0 of 3 reports by companies with hazard statement code(s).; Information may vary between notifications depending on impurities, additives, and other factors. The percentage value in parenthesis indicates the notified classification ratio from companies that provide hazard codes. Only hazard codes with percentage values above 10% are shown. For more detailed information, please visit ECHA C&L website. Source: European Chemicals Agency (ECHA)
- Sources/Uses: Azine dye; [Kirk-Othmer] Used to make inks and shoe-polish and for dyeing leather, wood, textiles, and plastics; [HSDB] Made from the reaction of nitrobenzene on aniline in the presence of iron(II) chloride; The crude mixture of nigrosine hydrochlorides (Phenol Black L) is used to dye phenolic resins; Bases are used to make printing colors and to dye plastics; [Ullmann] Source: Haz-Map, Information on Hazardous Chemicals and Occupational Diseases
- Industrial Processes with risk of exposure: Leather Tanning and Processing [Category: Industry]; Textiles (Printing, Dyeing, or Finishing) [Category: Industry] Source: Haz-Map, Information on Hazardous Chemicals and Occupational Diseases
- Uses: SHARK REPELLENT /NIGROSINE DERIV/ Source: Hazardous Substances Data Bank (HSDB)
- Uses: IN MANUFACTURE OF INK, SHOE-POLISH; IN DYEING LEATHER, WOOD, TEXTILES, PLASTICS Source: Hazardous Substances Data Bank (HSDB)
- Uses: IN SOAP, ANODIZED ALUMINUM, PAPER /SULFATED NIGROSINES/ Source: Hazardous Substances Data Bank (HSDB)
- Uses: NIGROSINE DYES ARE CONSTITUENTS OF LAUNDRY INDELIBLE INK Source: Hazardous Substances Data Bank (HSDB)
Mapped by: Existing checksum-valid CAS to unique PubChem CID match. Retrieved: 2026-08-31. Open source record
Scientific classifications and hazard annotations provide context and do not replace the market-specific regulatory decision shown above.
Additional source coverage and match status
| Source | Result | Checked |
|---|---|---|
| EMBL-EBI ChEBI | Exact match | 2026-08-31 |
| NIH PubChem PUG-View | Exact match | 2026-08-31 |
A recorded no-match is useful evidence that the source was checked; it is not a claim that the substance does not exist elsewhere.
Retrieved from NIH PubChem on 2026-08-31 by checksum-valid CAS matching. Chemical properties do not replace the regulatory decision on this page.